Arrowsmith, Cheryl (Ontario Cancer Institute)
Burrow, Tim (CSICOMP, Chemistry)
Forman-Kay, Julie (SickKids)
Ikura, Mitsui (Medical Biophysics UofT, Ontario Cancer Institute)
Kanelis, Voula (Chemical & Physical Sciences, Mississauga)
Kay, Lewis (Biochemistry)
Macdonald, Peter (Chemical & Physical Sciences, Mississauga)
Reynolds, William (Chemistry)
Seabrook, Genevieve (NMR Facility, Ontario Cancer Institute)
Sharpe, Simon (SickKids)
Simpson, Andre (Chemistry, Scarborough)
Simpson, Myrna (Environmental Science, Scarborough)
Soong, Ronald (Environmental NMR Center, Scarborough)
Associate, NMR, Department of Chemistry, University of Toronto
Associate (Limited Term) - Nuclear Magnetic Resonance (NMR) Spectroscopy
Job Number: 1800465
Faculty / Division: Faculty of Arts and Science
Campus: St. George (downtown Toronto)
Description: The Department of Chemistry in the University of Toronto invites applications for a Research Associate (Limited Term) for an eighteen month appointment. The anticipated start date is April 15, 2018.
The NMR Facility in the Department of Chemistry has a total of ten NMR spectrometers, ranging in field strength from 200 to 700 MHz as well as an EPR spectrometer. Some systems are used in a walk-up fashion, while sample changers are used to facilitate most of the routine NMR acquisitions. The Facility has both Agilent/Varian and Bruker spectrometers with cryogenically cooled probes, solids probes, and a wide range of liquids probes.
The incumbent will be under the general direction of the Facility Manager and will provide scientific and technical support services, with a focus on NMR spectroscopy and some EPR spectroscopy. Specifically, the incumbent will be responsible for or will assist with the following principal duties in the facility: implementing NMR experiments, client services, instrument maintenance, training, and developing operational workflow within the lab. The incumbent will also be expected to take a lead role in the implementation of solid state NMR, protein NMR, and EPR at the facility.
Job duties may include:
Implementing NMR and EPR experiments
-keeping abreast of current trends in experimental NMR and EPR by maintaining familiarity with current literature
-developing a knowledge-base to effectively assess a diverse range of samples using a variety of NMR and EPR experiments
-participating in external training and courses
-performing specialized experiments on the CSICOMP NMR instrumentation, particularly solid state NMR, protein NMR, and EPR
-implementing new NMR techniques on the CSICOMP instrumentation
-preparing and testing macros for the vendor software to enhance the instrument performance and user experience
-developing NMR-related research in collaboration with facility users
-corresponding with and assisting research personnel in this and other universities to provide expert opinion on NMR techniques, sample handling, spectrometer use, NMR processing, and data interpretation
-determining the feasibility of and performing specialized experiments on the CSICOMP NMR instrumentation, particularly solid state NMR and EPR
- regularly performing routine calibrations and adjustments on the spectrometers and related accessories
- assisting with troubleshooting and spectrometer repair, including related hardware, software and operating system (i.e. Linux) maintenance and management
- assisting with cryogen fills
- updating and maintaining teaching pedagogy
- scheduling training sessions and corresponding with new users
- evaluating student competencies
- assisting with new-user training
operational workflow within the CSICOMP facility
- assisting with sample-tracking, record keeping, billing, and other associated paperwork for the NMR accounts
- developing and maintaining online notes and documentation that relate to troubleshooting, maintenance, and repair efforts
- testing, utilizing, and maintaining familiarity with CSICOMP standard operating procedures (SOPs)
Ph.D. in the field of chemistry, physics, or biochemistry.
Minimum two years experience in an NMR laboratory environment with at least one year of experience maintaining NMR instrumentation
Experience with solid state NMR, including calibrations, sample preparation and basic experimental techniques; advanced experience with solid state NMR acquisition, processing, and analysis techniques and related instrumentation are preferred
Experience with 1D and 2D solution state NMR techniques, including sample preparation, experimental methodology, data processing, and interpretation; experience with protein NMR techniques (experimentation and data processing) is preferred
Experience with NMR spectrometers and associated hardware including automatic sample changers
Experience handling cryogens, compressed gases, dewars, and gas cylinders
Experience with EPR techniques, along with related instrumentation is an asset
Experience with common spectrometer and magnet maintenance, such as cryogen fills, probe changes, are an asset
Teaching and training experience is an asset
Demonstrated communication and interpersonal skills
Demonstrated ability to work in an customer focused environment with changing priorities, unanticipated urgent demands and frequent interruptions
Proficiency with Outlook, Word and Excel
Basic knowledge of computer operations including vendor-packaged software
Experience with Unix and/or is Linux is an asset
Demonstrated proficiency in the field of NMR spectroscopy as indicated by peer-reviewed publication and/or conference presentations
Ability to troubleshoot technical problems related to the spectrometers, associated hardware, and software. This includes operating system (i.e. Linux) maintenance and management
Applicants should apply online through the following link:
Please include a covering letter, curriculum vitae, and three reference names with their contact addresses and phone numbers by the closing date of March 16, 2018. Any questions regarding the position should be directed to Darcy Burns at [ darcy.burns "at" utoronto.ca ].
All qualified candidates are encouraged to apply; however, Canadians and permanent residents will be given priority.
Employee Group: Research Associate, Limited Term
Appointment Type: Grant - Term
Pay Scale Group and Hiring Rate: R01 -- Research Associates (Limited Term): $43,342 - $81,266
Job Field: Research Associate (Limited Term)
Job Posting: Feb 9, 2018
Job Closing: Mar 16, 2018, 10:59:00 PM
The University of Toronto is strongly committed to diversity within its community and especially welcomes applications from racialized persons / persons of colour, women, Indigenous / Aboriginal People of North America, persons with disabilities, LGBTQ persons, and others who may contribute to the further diversification of ideas.
part of your application, you will be asked to complete a brief Diversity
Survey. This survey is voluntary. Any information directly related to you
is confidential and cannot be accessed by search committees or human resources
staff. Results will be aggregated for institutional planning purposes. For
more information, please see http://uoft.me/UP
Environmental NMR Centre, Department of Chemistry, University of Toronto at Scarborough, Toronto, Ontario, Canada
The project will, in collaboration with Bruker BioSpin Corporation and the Ontario Ministry of the Environment and Climate Change aim to develop automated platforms to asses and understand metabolic change as a result of environmental stress (contaminants, temperature, salts, etc.) based on digital microfluidics interfaced with NMR.
The candidate will be involved in a range of development, including
1) In collaboration with Bruker assist in the development of new micro-coils.
2) Developing NMR methods to narrow line shape in static environmental and biological samples on micro-coils.
3) In collaboration help interface the micro-coils to digital micro-fluidics devices.
4) Test and develop protocols for exposing in-vivo organisms and eggs (including fully labelled 13C and 15N organisms/eggs) to stressors and interpreting the metabolic response.
The candidate will be based in the Environmental NMR Center at the University of Toronto. The candidate will have access to a range of state of-the-art equipment at the Environmental NMR center including prototype Comprehensive Multiphase NMR probes (CMP-NMR) (J. Magn. Reson. 2012; 217:61-76), a range of unique micro coil hardware, as well as solid-state, HR-MAS, liquid-state, cryoprobes, micro-imaging, and hyphenated (2D-HPLC-SPE-NMR-MS(Q-q-TOF) NMR spectrometers.
The candidate will be expected to spend time at Bruker (U.S.) and Bruker (Switzerland) and the Ontario Ministry of the Environment and Climate Change (Burlington, Canada). The project aims to bring together industry, academia and government and the postdoc will be expected play a central role in extending this collaboration.
Often, numerous environmental contaminants are found at low levels which in isolation are non-lethal but combined exhibit toxicities that are difficult to evaluate. In the long term they may be very hazardous to animal, plant and human populations, as their affects are often detected too late, and after physical symptoms become widespread. This project will develop in-vivo NMR that will permit molecular fingerprinting approaches that directly measure the changes in a living organism as a direct response to its surroundings. This research aims to develop tools that can answer the key question Is a particular contaminated environment safe for life? and understand how and why certain chemicals are toxic. Preliminary results demonstrate the approaches can efficiently and quantitatively asses stress in natural populations months/years before conventional reproduction tests, as-well as explaining the source of the stress and its biochemical implications. Furthermore, such early warning systems could potentially being used to predict and permit treatment of disease at its very early stages before symptoms are apparent.
The project is funded for a 3 year period. Preference will be given to candidates that can commit to at least 2 years (ideally 3 years). Candidates must be available to start Jan 1st 2017 (at the latest).
The candidate must have a strong background in NMR spectroscopy, experience with pulse sequence development, micro-coil NMR, isotopically enriched NMR, and experience with heterogeneous samples is an asset. The candidate must be open minded and willing to work with very complex in-vivo systems. The candidate should be willing to learn to design novel pulse programs that combine aspects from solution-state and solid-state NMR. Candidates will need to draw upon and integrate a range of concepts including saturation transfer, diffusion editing, cross-polarization dynamics, relaxation filters, isotope filtering with 1-3D NMR spectroscopy to access specific key molecular interactions from within complex matrices.
The candidate should have an interest in environmental chemistry and/or willingness to learn key issues in this field. Due to the very technical nature of the project the position is specifically suited to a gifted and open-minded NMR spectroscopist rather than an environmental scientist with NMR experience. In addition, the candidate, along with the center manager and directors, will be expected to act as a general NMR resource for graduate students, and collaborators in the Environmental NMR Centre, and assist with training, data acquisition, processing and interpretation on projects as required. The Environmental NMR Centre is highly collaborative and such involvement will nearly always result in the post-doctoral fellow becoming a co-author in any research in which they assisted. We expect the candidate lead at least two first author publications per year.
Candidates should send a complete C.V. including a publication list, a statement outlining their suitability and their interest in the position. Candidates should arrange to have 3 references sent directly. Applications without the appropriate references have to be considered incomplete and cannot be considered.
Applications and references should be sent to
André Simpson, FRSC
Director of the Environmental NMR Center
Department of Chemistry
Division of Physical and Environmental Sciences
University of Toronto at Scarborough
1265 Military Trail
Toronto, MIC 1A4
e-mailed as a PDF attachment to
andre.simpson "at" utoronto.ca
All complete applications and references will be reviewed upon submission. The position is available immediately and will remain open until filled.
Professor Andre Simpson, PhD, FRSC
of the Environmental NMR Center, Director of TRACES
Department of Chemistry
University of Toronto, Scarborough Campus
1265 Military Trail
Andre J. Simpson, "Editorial: Environmental NMR," Magnetic Resonance in Chemistry 53 (2015) 633-634. http://dx.doi.org/10.1002/mrc.4241
Caroline M. Preston, "Environmental NMR – the early years," Magnetic Resonance in Chemistry 53 (2015) 635-647. (historical perspective) http://dx.doi.org/10.1002/mrc.4180
Myrna J. Simpson (Editor)
Wiley: The challenges faced by environmental scientists today are vast, complex, and multi-faceted. For instance, predicting the fate of an environmental pollutant or understanding ecosystem responses to climate change, necessitate a firm understanding of molecular structure and dynamics of environmental media as well as the components that exist and interact within this media. Furthermore, linking information obtained at the molecular-scale to ecosystem-level processes is a major pursuit of modern environmental research. As such, NMR spectroscopy and its scalability from the molecular-scale to the macroscopic-scale, is facilitating rapid growth in environmental science. In addition, the versatility of NMR spectroscopy has resulted in the development and implementation of different types of NMR techniques to examine the structure of various types of environmental samples, living and non-living, as well as the study of critical environmental processes.
This comprehensive handbook is a collection of chapters that span from methods to how NMR is used in environmental research to gain insight into various ecosystem properties. It is organized into three parts:
Part A focuses on methods used in environmental NMR which span from solution-state to magnetic resonance imaging.
Part B emphasizes how NMR spectroscopy plays an essential role in understanding various types of environmental components and related processes, including different forms of organic matter found in soil, water, and air as well as how NMR is used to probe the fate of water, organic pollutants, and metals in the environment.
Part C focuses on the growing field of environmental metabolomics which uses NMR as its main discovery platform.
This volume highlights the immense potential of NMR spectroscopy to expand our fundamental understanding of environmental processes and how it will continue to do so well into the future.
Blaine Chronik (Western) Magnetic Resonance Systems Development
Kalle Gehring (McGill) Protein folding in the endoplasmic reticulum
Paul Hazendonk (Lethbridge) Solid-State NMR of Complex Hybrid Organic-Inorganic Materials
William MacFarlane (UBC) Development and Application of Depth-Resolved beta-Detected Nuclear Magnetic Resonance to electronic, ionic and molecular phenomena in the Solid State
Anthony Mittermaier (McGill) Binding Mechanisms and Conformational Equilibria in Biomacromolecular Interactions
Geoffrey Ozin (Toronto) Silicon Nanochemistry
Linda Reven (McGill) Solid-State NMR of Polymer and Liquid Crystalline Nanocomposites
Andre Simpson (Toronto Scarborough) Development of flow-based and magic angle spinning in-vivo NMR to understand environmental stress
Jenifer Thewalt (Simon Fraser) Domains in Biologically Relevant Lipid Membranes
Julian Zhu (Université de Montréal) Versatile Polymeric Biomaterials
Discovery Accelerator Supplement
$120,000 over three years
|Giuseppe Melacini (McMaster) Solution NMR Studies of Interactions of Ligands With Plasma Proteins|
Research Tools and Instruments (RTI)
|Derek Gates (UBC) Upgrade to UBC-Chemistry Departmental NMR Facility|
A. Sutrisno, A.J. Simpson, "Metals in the Environment," eMagRes 2 (2013) 467–476. http://dx.doi.org/10.1002/9780470034590.emrstm1347
P.J. Mitchell, A.J. Simpson, M.J. Simpson, "Dissolved Organic Matter," eMagRes 2 (2013) 503–516. http://dx.doi.org/10.1002/9780470034590.emrstm1348
S. Booth, R.J. Turner, A. Weljie, "Metabolomics in Environmental Microbiology," eMagRes 2 (2013) 517–528. http://dx.doi.org/10.1002/9780470034590.emrstm1335
M.J. Simpson, D.W. Bearden, "Environmental Metabolomics: NMR Techniques," eMagRes 2 (2013) 549–560. http://dx.doi.org/10.1002/9780470034590.emrstm1352
S.A. Quideau, C.E. Norris, L.N. Soucémarianadin, R.E. Wasylishen, "Forest Ecology and Soils," eMagRes 2 (2013) 597–608. http://dx.doi.org/10.1002/9780470034590.emrstm1339
A.J. Simpson, D. Courtier-Murias, J.G. Longstaffe, H. Masoom, R. Soong, L. Lam, A. Sutrisno, H. Farooq, M.J. Simpson, W.E. Maas, M. Fey, B. Andrew, J. Struppe, H. Hutchins, S. Krishnamurthy, R. Kumar, M. Monette, H.J. Stronks, "Environmental Comprehensive Multiphase NMR," eMagRes 2 (2013) 399–414. http://dx.doi.org/10.1002/9780470034590.emrstm1337
W.L. Meerts, C.A. de Lange, A.C.J. Weber, E.E. Burnell, "Analysis of Complex High-Resolution NMR Spectra by Sophisticated Evolutionary Strategies," eMagRes 2 (2013) 437–450. http://dx.doi.org/10.1002/9780470034590.emrstm1309
Cover article in the Journal of the American Chemical Society (June 28/13)
Algirdas Velyvis and Lewis E. Kay, "Measurement of Active Site Ionization Equilibria in the 670 kDa Proteasome Core Particle Using Methyl-TROSY NMR," J. Am. Chem. Soc. 135 (2013) 9259–9262. (cover article) http://dx.doi.org/10.1021/ja403091c
paper in Science
P. Neudecker, P. Robustelli, A. Cavalli, P. Walsh, P. Lundström, A. Zarrine-Afsar, S. Sharpe, M. Vendruscolo, and L.E. Kay, "Structure of an Intermediate State in Protein Folding and Aggregation," Science 336 (2012) 362-366. http://dx.doi.org/10.1126/science.1214203
Science Perspective: David Eliezer, "Visualizing Amyloid Assembly," Science 336 (2012) 308-309. http://dx.doi.org/10.1126/science.1220356
introductory lecture on physics of MRI by Donald Plewes
and Walter Kucharczyk (University of Toronto).
Our keynote lecturer this year is Prof. Chad Reinstra (UIUC) on BioSolids NMR. We will have Dr. Frank Delaglio (NIDDK, NIH) for the NMRPipe Suite; Dr. Nico Tjandra (LBPC, NIH) with Dynamics and Relaxation; Dr. Bruce Johnson (UMBC) on NMRView; Dr. Charles Schwieters (ISL, NIH) demonstrating Xplor-NIH, and one day on pulse sequence programming (Agilent/Bruker).
more information visit
Please mark your calendars,
Timothy Burrow, Ph.D. MBA
University of Toronto 416-978-5728
NMR Web pages:
Special issue of the Journal of Biomolecular NMR on the occasion of Prof. Lewis E. Kays 50th birthday, volume 51, numbers 1-2, September 2011.
Kevin H. Gardner, Anthony Mittermaier and Frans A.A. Mulder, "A tribute to Lewis E. Kay on his 50th birthday" Journal of Biomolecular NMR 51 (2011) 3-4. (Editorial) http://dx.doi.org/10.1007/s10858-011-9561-x
A fitting collection of 18 research publications by colleagues, friends and former students of Lewis Kay to celebrate his many pioneering contributions to the field of biomolecular NMR spectroscopy.
J.R. Bothe, E.N. Nikolova, C.D. Eichhorn, J. Chugh, A.L. Hansen and H.M. Al-Hashimi, "Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy," Nature Methods 8 (2011) 919931. (Review) http://dx.doi.org/10.1038/nmeth.1735
H.N. Cheng, T. Asakura, A.D. English
This ACS Symposium Series book includes papers presented at the Symposium "NMR Spectroscopy of Polymers - Innovative NMR Strategies for Complex Macromolecular Systems" which was part of Pacifichem 2010, the 2010 International Chemical Congress of Pacific Basin Societies in Honolulu, Hawaii, December 15-20, 2010. Among over 30 contributions on the state-of-the-art NMR spectroscopy in polymers and related systems there are two chapters by Canadian NMR research groups.
P.M. Macdonald, "Diffusion NMR of Polymers in Bicelles," Chapter 14, NMR Spectroscopy of Polymers: Innovative Strategies for Complex Macromolecules, Eds. H.N. Cheng, T. Asakura, A.D. English, ACS (2011) pp. 221-250. http://dx.doi.org/10.1021/bk-2011-1077.ch014
H. Thérien-Aubin, Y.J. Wang, and X.X. Zhu, "NMR Imaging and Its Application in the Study of Pharmaceutical Tablets," Chapter 27, NMR Spectroscopy of Polymers: Innovative Strategies for Complex Macromolecules, Eds. H.N. Cheng, T. Asakura, A.D. English, ACS (2011) pp. 441-457. http://dx.doi.org/10.1021/bk-2011-1077.ch027
G. Bouvignies, P. Vallurupalli, D.F. Hansen, B.E. Correia, O. Lange, A. Bah, R.M. Vernon, F.W. Dahlquist, D. Baker & L.E. Kay, "Solution structure of a minor and transiently formed state of a T4 lysozyme mutant," Nature 477 (2011) 111114. http://dx.doi.org/10.1038/nature10349
T. Wu, H. Mayaffre, S. Krämer, M. Horvatic, C. Berthier, W.N. Hardy, R. Liang, D.A. Bonn and M.-H. Julien, "Magnetic-field-induced charge-stripe order in the high-temperature superconductor YBa2Cu3Oy," Nature 477 (2011) 191194. http://dx.doi.org/10.1038/nature10345
Cambridge Isotope Laboratories (CIL) made available a series of application notes on the use of stable isotopes in Biomolecular NMR. These application notes were written by some of the world's leading researches in the field, including several Canadian scientists.
Lewis Kay (University of Toronto) "Alanine Probes of Supra-Molecular Structure and Dynamics", page 9.
Leonid S. Brown and Vladimir Ladizhansky (University of Guelph) "Pichia pastoris as a Eukaryotic Protein Isotope-Labeling System", pages 14-15.
Ying Fan, Lichi Shi, Vladimir Ladizhansky and Leonid S. Brown (University of Guelph) "Uniform Isotope Labeling of Eukaryotic Proteins in Methylotrophic Yeast for High-Resolution NMR Studies - Extension to Membrane Proteins", Application Note 26, pages 71-74.
Recent research by Gang Wu's group (Queen's University) is cited as an example of advanced 17O NMR spectroscopy in large protein-ligand complexes in solution and in the solid state, "17O NMR reagents", page 34.
application notes are available for download as a PDF file (5.1MB) "Stable
Isotopes for Structural Biomolecular NMR Catalog" at:
Myrna Simpson (University of Toronto) has been promoted to the rank of Full Professor (Environmental Chemistry) starting July 1, 2011.
Myrna received a BSc (Chemistry & Mathematical Sciences) and a PhD (Environmental Soil Chemistry) from the University of Alberta. Myrna joined the University of Toronto in 2002 after a 2.5 year postdoctoral fellowship with Prof. Patrick Hatcher in the Department of Chemistry at the Ohio State University.
Myrna's research in environmental and analytical chemistry involves the development and application of molecular-level analytical tools to improve the fundamental understanding of soil environmental processes. In particular her group is using advanced mass spectrometry (MS) and nuclear magnetic resonance (NMR) methods to characterize, monitor and predict the fate of soil organic matter components in various environments. In 2010 she was awarded the SETAC/Royal Society of Chemistry Award in Environmental Science for "outstanding contributions that have advanced the understanding or development of environmental systems, technologies, methodologies or other relevant research in the environmental sciences".
In addition to teaching and research, Myrna acts as the Associate Director of the Environmental NMR Centre of the Department of Physical and Environmental Sciences, the University of Toronto.
For more information visit: http://www.utsc.utoronto.ca/~msimpson
Photo credit: University of Toronto
|J.L. Kitevski-LeBlanca and R.S. Prosser, "Current Applications of 19F NMR to Studies of Protein Structure and Dynamics," Progress in Nuclear Magnetic Resonance Spectroscopy (2011) accepted. (Invited Review) http://dx.doi.org/10.1016/j.pnmrs.2011.06.003|
André Simpson (Department of Chemistry, University of Toronto at Scarborough) has been awarded the 2011 CSC W.A.E. McBryde Medal for his research in the development of nuclear magnetic resonance (NMR) spectroscopy with the specific objective to address environmental problems at a molecular level.
From the citation: "Andre Simpson obtained his PhD from the University of Birmingham, U.K. then moved to the U.S. completing two postdoctoral fellowships, first at Mississippi State and then at Ohio State University. Simpson is an associate professor at the University of Toronto. He is best known for his research developing nuclear magnetic resonance-based methods to study the structure and interactions in complex environmental mixtures. His research has helped understand how and why different carbon pools respond to climate change and unravel the complexities of contaminant fate and toxicity in the environment. Simpson has published more than 60 refereed articles since his first journal publication in 2000 and contributed to 12 book chapters. In 2004, he co-founded the Environmental NMR Centre at the University of Toronto, a first of its kind in Canada. In 2008 he was ranked in the Ten to Watch for in 2008 by the Toronto Star. Later in the same year he was awarded the Royal Society of Chemistry/Society for Environmental and Toxicology and Chemistry Environmental Science Award, a global award recognizing outstanding contributions to the field."
the announcement at
The Department of Chemistry at the University of Toronto has ordered $5.5M of new NMR spectrometers from Agilent Technologies to equip its new 4,000-square-foot NMR laboratory. Dr. Timothy Burrow, Manager of the NMR Facility, informed that the new spectrometers will be a 500 MHz spectrometer, with 7600AS sample changer and XSens 13C sensitive cryogenically cooled probe, a 600 MHz dual liquids and solids spectrometer and a 700 MHz spectrometer with a H/F,CN Cold Probe, 7600AS sample changer and solids, biosolids and semi-solids probes.
"These new NMR systems will provide a vital boost to our new Centre for Spectroscopic Investigation of Complex Organic Molecules and Polymers (CSICOMP), increasing the range and scope of research in key areas," said Professor Robert H. Morris, Chair of the Chemistry Department.
The new facility is scheduled to open later this year, the International Year of Chemistry. More than 45 scientists as well as 300 graduate students and postdoctoral fellows will be able to use the facility for inorganic, organic, materials and bio-organic research, including broader investigations into the fate of fluorinated compounds in the environment. The new facility is supported by the Canada Foundation for Innovation and the Ontario Ministry of Research and Innovation. (See related story in "Canadain NMR Research" News Bulletin, #3.3, Summer 2009, page 3, download)
"We are excited that the University of Toronto's new facility has chosen Agilent as its primary NMR equipment provider," said Kevin Meldrum, director, research products marketing for Agilent. "Agilent is dedicated to helping scientists meet all of their spectroscopy research needs and this agreement further illustrates that commitment."
the press release by Agilent Technologies
One of the textbook concepts in molecular biology is that proteins fold up spontaneously to form the most energetically stable three-dimensional structures. These folded proteins are presumed structurally rigid, which is important for their unique functionality, e.g. as enzymes. There are indications, however, mostly coming from NMR studies, that many important proteins exist in disordered form. A news feature in the latest issue of Nature discusses implications of this potentially far-reaching finding. Among cited examples of intrinsically disordered proteins is the NMR study on Sic1 protein by Julie Forman-Kay and her group at the University of Toronto/SickKids.
T. Chouard "Structural biology: Breaking the protein rules," Nature 471 (2011) 151-153. http://dx.doi.org/10.1038/471151a
January 17th 2011, Dr. Mitsu Ikura received the 2010 Canadian
Cancer Society "Robert L. Noble Prize" for his outstanding
contributions to cancer research in Canada.
From the citation (http://www.cancer.ca): "Dr. Ikura is an internationally recognized authority in the field of structural biology and has laid the groundwork for our understanding of signalling proteins such as cadherins and catenins and molecular signalling processes involved in human diseases such as cancer. His studies also provide excellent platforms for developing new drugs designed to interfere with the functioning of cancer cells.
Dr Ikura is a senior scientist at the Ontario Cancer Institute and a professor at the University of Toronto. He received his PhD in macromolecular biophysics from Hokkaido University, Japan and pursued postdoctoral studies on multi-dimensional NMR spectroscopy of a calmodulin-kinase peptide complex at the National Institutes of Health.
Dr Ikura has a Tier-1 Canada Research Chair in cancer structural biology and has been recognized by many awards and prizes including the William E. Rawls Prize, the International Research Scholar Award Howard Hughes Medical Institute, and the Premiers Research Excellence Award.
He has published over 190 peer-reviewed papers and has been invited to speak at more than 200 international scientific conferences."
About "Robert L Noble" Prize: The Robert L. Noble Prize is given for outstanding achievements in cancer research. It honours Dr Noble, an esteemed Canadian investigator whose research in the 1950s led to the discovery of vincristine, a widely-used anti-cancer drug. At the time, vincristine was one of the most effective treatments available for Hodgkins disease.
Web: the Ikura Laboratory http://nmr.uhnres.utoronto.ca/ikura/index.html
Recognition: André Simpson (Nov 23/10)
André Simpson, Professor of chemistry at the University of Toronto Scarborough, has been elected a Fellow of the Royal Society of Chemistry (RSC) (full story at UTSC).
|A.J. Simpson, D.J. McNally, M.J. Simpson "NMR Spectroscopy in Environmental Research: From Molecular Interactions to Global Processes," Progress in Nuclear Magnetic Resonance Spectroscopy (2010) in press. (Invited Review) http://dx.doi.org/10.1016/j.pnmrs.2010.09.001|
Kay, "Structure and Dynamics of Proteins Big and Small",
Encyclopedia of Magnetic Resonance (2010). http://dx.doi.org/10.1002/9780470034590.emrhp1027
W.F. Reynolds, "Heteronuclear Multiple Bond Correlation (HMBC) Spectra", Encyclopedia of Magnetic Resonance (2010). http://dx.doi.org/10.1002/9780470034590.emrstm1176
D.M. Korzhnev, T.L. Religa, W.Banachewicz, A.R. Fersht, L.E. Kay, "A
Transient and Low-Populated Protein-Folding Intermediate at Atomic Resolution",
Science 329 (2010) 1312-1316. http://dx.doi.org/10.1126/science.1191723
Abstract: "Proteins can sample conformational states that are critical for function but are seldom detected directly because of their low occupancies and short lifetimes. In this work, we used chemical shifts and bond-vector orientation constraints obtained from nuclear magnetic resonance relaxation dispersion spectroscopy, in concert with a chemical shiftbased method for structure elucidation, to determine an atomic-resolution structure of an "invisible" folding intermediate of a small protein module: the FF domain. The structure reveals non-native elements preventing formation of the native conformation in the carboxyl-terminal part of the protein. This is consistent with the kinetics of folding in which a well-structured intermediate forms rapidly and then rearranges slowly to the native state. The approach introduces a general strategy for structure determination of low-populated and transiently formed protein states."
This research article by Lewis Kay (University of Toronto) and colleagues is also accompanied by the Science Perspective
H.M. Al-Hashimi, "Exciting Structures", Science 329 (2010) 1295-1296. http://dx.doi.org/10.1126/science.1195571
Wiley: "Multidimensional NMR methods have transformed the way in which solution state NMR is used to elucidate the structures of chemical and biochemical systems. The first book covering new developments in nearly a decade, this much-needed resource explains recent experimental methods for the rapid measurement of multidimensional solution-state NMR spectra. With articles written by key developers of the techniques, the coverage deals with both the theoretical tools and the latest practical applications, giving an unmatched guide to students, researchers, technicians, and anyone else working with NMR techniques today"
A.D. Bain "COSY: Quantitative Analysis," Chapter 13, Multidimensional NMR Methods for the Solution State (EMR Books), Eds. G.A. Morris and J.W. Emsley, Wiley (2010) pp. 167-176. http://dx.doi.org/10.1002/9780470034590.emrstm0095
T.T. Nakashima and R.E.D. McClung "Heteronuclear Shift Correlation Spectroscopy," Chapter 22, Multidimensional NMR Methods for the Solution State (EMR Books), Eds. G.A. Morris and J.W. Emsley, Wiley (2010) pp. 289-304. http://dx.doi.org/10.1002/9780470034590.emrstm0209
R. Muhandiram and L.E. Kay "3D HMQC-NOESY, NOESY-HMQC, and NOESY-HSQC," Chapter 25, Multidimensional NMR Methods for the Solution State (EMR Books), Eds. G.A. Morris and J.W. Emsley, Wiley (2010) pp. 335-350. http://dx.doi.org/10.1002/9780470034590.emrstm0563
The complete Table of Contents is available on Stan's NMR blog.
Burrow (University of Toronto) wrote a utility application for the
iPhone/iPod touch that calculates attenuation values:
Attenuator is a utility for anyone working with radio frequency sources and needs to know power and voltage after attenuation.
You can specify an input voltage (Vpp or Vrms) and dB of attenuation to get the output voltage, power and attenuation. The impedance can be specified, typically 50 Ohm for most systems.
Natural Sciences and Engineering Research Council of Canada (NSERC) has announced results of the 2010 NSERC competition in the Discovery Grants Program (DG), Research Tools and Instruments Grants (RTI) and Scholarship programs. Among grant recipients
Myrna Simpson's (University of Toronto Scarborough) NSERC Discovery Grant was renewed. Myrna has also been awarded an NSERC Discovery Accelerator Supplement.
Share your success with the Canadian NMR community (E-mail).
The Canada Foundation for Innovation (CFI) has announced today major investment in Canadian Research Infrastructure. More than $665 million was awarded to 133 projects at 41 Canadian research institutions through the CFIs Leading Edge Fund and New Initiatives Fund. Among infrastructure projects receiving Government's support is the Canada's first 950 MHz NMR spectrometer at the University of Toronto (Lewis Kay, Biochemistry) to enable studies of the structure and dynamics of complex biological molecules, as well as the technological development of biological NMR.
Project title: "High Field NMR Studies of Protein Molecules in Health and Disease" (CFI contribution $4,595,843)
This very significant hardware acquisition will ensure Canada's leading role
in biological NMR research.
Congratulations are due to Lewis Kay and his team at the UofT !
A complete list of awarded projects (CFI web-site)
Lewis Kay and colleagues from the University of Toronto have published an article in Biophysical Journal reviewing recently developed NMR relaxation dispersion technique and its applications to protein folding research.
Neudecker, P. Lundström, L.E. Kay, "Relaxation Dispersion NMR
Spectroscopy as a Tool for Detailed Studies of Protein Folding," Biophysical
Journal 96 (2009) 2045-2054. (review)
This review and all previous issues of Biophysical Journal are available free of charge until April 5, 2009. The free access is provided by Cell Press, who is taking over publishing Biophysical Journal starting in 2009.
A research team from the Ontario Cancer Institute and the University of Toronto has developed a real-time, nuclear magnetic resonance (NMR)based assay to monitor the intrinsic GTPase activity of the small guanosine triphosphatase (GTPase) Rheb. This method can now be used to gain further understanding of the mechanisms of action of other GTPases and their GAP partners.
C.B. Marshall, J. Ho, C. Buerger, M.J. Plevin, Guang-Yao Li, Z. Li, M. Ikura, and V. Stambolic, "Characterization of the Intrinsic and TSC2-GAPRegulated GTPase Activity of Rheb by Real-Time NMR," Science Signaling 2 (#55) (2009) ra3. http://dx.doi.org/10.1126/scisignal.2000029
Science Signaling is a weekly AAAS journal as well as an online resource and information management tool that enables experts and novices in cell signaling to find, organize, and utilize information relevant to processes of cellular regulation. As of September 2008, Science Signaling is adding original research articles to the weekly journal. Science Signaling publishes research that represents a major advance in cell signaling, including key research papers in the rapidly expanding areas of signaling networks, systems biology, synthetic biology, computation and modeling of regulatory pathways, and drug discovery.
about Science Signaling:
A research team from the University of Toronto Scarborough led by Myrna Simpson reports an accelerated rate of decomposition of some soil organics and accumulation of others due to soil warming.
X. Feng, A.J. Simpson, K.P. Wilson, D.D. Williams and M.J. Simpson, "Increased cuticular carbon sequestration and lignin oxidation in response to soil warming," Nature Geoscience (2008) online. http://dx.doi.org/10.1038/ngeo361
This paper has generated considerable media attention including a feature interview "Soil Alert" with Myrna on Daily Planet (aired on Nov 27, 2008).
web : Myrna Simpson's Group (external link)
A Chemical Approach to Nanomaterials
Geoffrey A. Ozin, Andre C. Arsenault and Ludovico Cademartiri
Hardcover: 770 pages
Publisher: Royal Society of Chemistry; 2nd Revised edition (January 2009)
RSC: The global success of the 1st edition of "Nanochemistry", and exceptionally rapid change in the field, has necessitated the publication of a 2nd edition after only three years. This truly major update highlights the latest breakthroughs using over 80 new case histories, more problem sets, and more teaching principles. Written for teachers and students, the book catapults the reader to the forefront of the field. Using simple language, and focusing on the concepts, it covers all chemistry techniques commonly used to synthesize nanomaterials. In this book, case histories enable readers to 'connect the dots' and understand the possibilities ahead whilst problem sets encourage students to think creatively and laterally about what they have learnt. The extensive bibliography will satisfy those hungry for more detail.
web: Geoffrey Ozin (University of Toronto)